Cyclophosphamide Pharmacokinetics in Mice: A Comparison Between Retro Orbital Sampling Versus Serial Tail Vein Bleeding



Rana Saida, b, Mohamed Abdel-Rehimc, d, Behnam Sadeghia, b, Suleiman Al-Hashemia, b, Zuzana Hassan a, b, Moustapha Hassan a, b, *
a Department of Laboratory Medicine, Karolinska Institute, Stockholm, Sweden
b Experimental Cancer Medicine, Clinical Research Center, Karolinska University Hospital-Huddinge, Sweden
c Astrazeneca, RandD, Södertälje, DMPKBAC, 15185 Södertälje, Sweden
d Department of Chemistry, Karlstad University, 65188 Karlstad, Sweden


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© 2007 Said et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Experimental Cancer Medicine, Clinical Research Center, Novum, Karolinska University Hospital-Huddinge, S-141 86 Stockholm, Sweden; Tel: +46-8-58583862; Fax: +46-8-58583810; E-mail: moustapha.hassan@ki.se


Abstract

Preclinical studies on pharmacokinetics in animals usually require at least 3-5 animals per time point. The use of several animals in each time point may increase the result variation due to individual dosing errors and inter- and intraindividual variation among animals. Moreover, a large number of animals has to be euthanized in these experiments. In the present paper, the pharmacokinetics of the anticancer drug, cyclophosphamide was investigated using serial bleeding from the tail vein and compared with the kinetics obtained from traditional retro-orbital sinus bleeding in mice. Cyclophosphamide (100 mg/kg) was administered intraperitoneally to two groups of mice. Blood samples were collected at 0, 0.25, 0.5, 1, 2, 3, 4, 5 and 6 hr. In the first group (n=6), 20 µL blood samples were collected from the tail-vein by serial bleeding. In the second group, 3 animals were killed at each time point and blood was collected from both tail vein and retro-orbital sinus. Cyclophosphamide analysis in whole blood was carried out using LC-MS/MS with on-line sample preparation utilizing microextraction by packed sorbent (MEPS). Pharmacokinetics including AUC, tmax, Cmax and half – life were estimated using WinNonLin.

Cyclophosphamide concentrations in blood obtained after sampling through tail vein were close to those obtained after retro-orbital bleeding within the same animal. No significant differences in estimated pharmacokinetic parameters were found when serial tail vein bleeding was compared to retro-orbital bleeding. Our results indicate that serial sampling using tail vein in mice can be a good alternative to retro-orbital sampling method. The method is reliable, easy and can be used for early preclinical kinetic studies. However, more studies are needed to evaluate different drug categories.

Keywords: Cyclophosphamide, pharmacokinetics, retro-orbital bleeding, tail-bleeding, LC/MS/MS.